A Simple, Rapid, and Cost-Effective PCR Procedure for Detection of NUDT15 Gene Variants in Vietnamese Patients with Acute Lymphoblastic Leukemia

Abstract Objective The NUDT15 variants impact thiopurine dose selection in acute lymphoblastic leukemia patients. ability to rapidly detect is important clinical practice. This study aims develop a simple polymerase chain reaction (PCR) procedure for detecting Vietnamese Materials and Methods Sanger sequencing was used determine from 200 We designed primers optimized the PCR detection of wild-type variant alleles compared with results. Results inserted c.55_56insGAGTCG detected by differences size through conventional PCR. tetra-primer amplification refractory mutation system successful two variations, c.52G > A c.415C T. sensitivity specificity achieved 100% when Conclusion Our suitable replacing setting.